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By Eraldo Antonini, Luigi Rossi-Bernardi, Emilia Chiancone (Eds.)

The seriously acclaimed laboratory typical, Methods in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. considering 1955, each one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this present day - really a necessary book for researchers in all fields of lifestyles sciences

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46-47): 46 HEMOGLOBINS AND MYOGLOBINS [3] i R. L. Garlick and R. C. Terwilliger, Comp. Biochem. Physiol. B 57B, 177 (1977). 2 T. Svedberg and K. O. Pedersen, "The Ultracentrifuge," p. 358. Oxford Univ. Press (Clarendon), London and New York, 1940. s j. Roche, in "Studies in Comparative Biochemistry" (K. A. ), p. 62. Pergamon, Oxford, 1965. 4 R. E. Weber, Comp. Biochem. Physiol. 35, 179 (1970). 5 L. Waxman, J. Biol. Chem. 246, 7318 (1971). 6 j. B. Swaney and I. M. Klotz, Arch. Biochem.

Nobbs, H. C. Watson, and J. C. Kendrew,Nature (London) 209, 339 (1966). 24T. Takano,J. Mol. Biol. 110, 569 (1977). 5. 4% saturated ammonium sulfate solutions at room temperature. Sperm Whale Oxymyoglobin. 7 Seal Myoglobin. The terminal amino group of seal and horse myoglobin is glycine, whereas in sperm and finback whale it is valine. For this reason, and because the crystal form is different, it was of interest to determine the structure of seal myoglobin,z6 A saturated solution of ammonium sulfate was added to a salt-free solution of myoglobin, 5%, to the point of incipient turbidity.

Biochem. 54, 137 (1973). [2] PREPARATION OF MYOGLOBINS 29 1 hr against 1 liter of the same buffer. ). The dialysis tubing is boiled with 10-4 M EDTA and is then washed exhaustively with deionized water before use. All steps of the preparation are done at 4°. The lysate is removed from the dialysis bag and centrifuged at 10,000 g for 30 min. 0; the centrifugation process is repeated. 03. Elution is done with this buffer at no more than 160 ml/hr. The eluent is directed to a fraction collector by a three-way valve until the desired fraction emerges.

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