Download General Microbiology by Roger Y. Stanier, Edward A. Adelberg, John L. Ingraham PDF

By Roger Y. Stanier, Edward A. Adelberg, John L. Ingraham (auth.)

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Not all microorganisms able to grow on solid media necessarily give rise to well-isolated colonies. Certain motile flagellated bacteria (Proteus, Pseudomonas, for example) can rapidly spread over the slightly moist surface of a freshly poured plate. This can be prevented by the use of plates with well-dried surfaces, on which the cells are immobilized. Spirochetes and organisms that show gliding movement (myxobacteria, blue-green bacteria) can move over or through an agar gel, even when its surface is well dried.

Although plating methods for the isolation of viruses have been greatly extended in recent years, many of these organisms are most easily isolated by the use of liquid media. In the case of viruses, of course, a pure culture is never obtainable, since these organisms are obligate intracellular parasites; a two-membered culture, consisting of a specific virus and its biological host, represents the goal of purification for this microbial group. The simplest procedure of isolation in liquid media is the dilution method.

A petri dish containing nutrient agar was streaked with a suspension of bacterial cells. As a result of subsequent growth, each cell has given rise to a macroscopically visible colony. chemical absorption or combustion. For the more oxygen-sensitive anaerobes, a modification of the pour plate method, known as the dilution shake culture, is to be preferred. A tube of melted and cooled agar is inoculated and mixed, and approximately one-tenth of its contents is transferred to a second tube, which is then mixed and used to inoculate a third tube in a similar fashion.

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