By Wei Lin
Magnetotactic micro organism (MTB) synthesize intracellular nano-sized minerals of magnetite and/or greigite magnetosomes for magnetic orientation. They play very important roles in international iron biking and sedimentary magnetism, and feature a vast variety of power functions in either biotechnological and biomedical fields. although, as the majority of MTB in nature stay unculturable, our knowing of those particular micro organism continues to be really constrained. This thesis describes the advance of a singular method for successfully accumulating, purifying and characterizing uncultivated magnetotactic micro organism. the variety, genomic details and rock magnetic homes of assorted uncultivated MTB are investigated and characterised utilizing a mix of organic and geophysical equipment. the consequences will bring about a greater figuring out of the biogeography and biomineralization mechanisms of MTB in nature, and increase our wisdom of the contributions of MTB to biogeochemical cycles of components and sedimentary magnetism.
Dr. Wei Lin works on the Institute of Geology and Geophysics, chinese language Academy of Sciences, China
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Additional info for Diversity, Biomineralization and Rock Magnetism of Magnetotactic Bacteria
Konetzka, W. A. (1978). A novel method for the isolation and study of a magnetotactic bacterium. Archives of Microbiology, 119, 203–212. Moyer, C. , Tiedje, J. , Dobbs, F. , & Karl, D. M. (1996). A computer-simulated restriction fragment length polymorphism analysis of bacterial small-subunit rRNA genes: Efficacy of selected tetrameric restriction enzymes for studies of microbial diversity in nature? Applied and Environment Microbiology, 62, 2501–2507. , & Camper, A. K. (2007). Genotypic microbial community profiling: A critical technical review.
This result suggested that the enriched magnetotactic cocci from Yuandadu Park belonged to a homogeneous population. 4 Spatiotemporal Variation of MTB Communities from Lake Miyun MTB communities from two separate sites (MY8 and MY11) in Lake Miyun were compared over a 3 month period (in total six samples) (Lin and Pan 2010). 1. 64, 40 3 Diversity of Magnetotactic Bacteria Fig. 6 a–c In situ hybridization of magnetically enriched coccoid cells, and d–f negative control of clones of E. coli with the inserted 16S rRNA genes of the uncultured Magnetococcus sp.
2005b; Flies et al. 2005a; Simmons et al. 2007; Lin et al. 2008; Pan et al. 2008; Lin et al. 2009; Lin and Pan 2009b). 1). 17 program (available at http://www. org/). The forward primer, FMTCf (50 -TAAAGCCCTTTY AGTGGGAA-30 ), corresponds to positions 431-450 of Escherichia coli, and the reverse primer, FMTCr (50 -ACTKCAATCYGAACTGAGACGAGY-30 ), corresponds to positions 1360–1383 of E. coli. The primer FMTCf was a modification of the primer MCF (Thornhill et al. 1995), which substituted the C at position 12 for Y, thus facilitating targeting more sequences of freshwater magnetotactic cocci.