By Ronne Randall
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The technique of making a smear should be practiced to produce good, readable smears for personal use or to submit to a consultant. Airdried blood smears must be made when the blood is collected to avoid cell deterioration. They should be mailed with the EDTA blood to a referral laboratory. One makes blood smears by placing a small drop of blood on one glass slide and using another spreader slide to make the smear. The spreader slide should be drawn back across the slide going through the drop of blood.
Two common veterinary instruments are the Bayer Advia 120 (Bayer H-1* is an earlier model being phased out of use) and the Abbott Cell-Dyn 3500†. The Cell-Dyn uses impedance technology for RBCs and platelets (see Figure 2-3). These provide abundant information about each cell type. The RDW describes the variability in size of the RBCs; the HDW describes variability in Hgb concentration among cells (see Chapter 3). , Tarrytown NY. † Abbott Cell-Dyn 3500, Abbott Diagnostics, Abbott Park, IL. 23 The Complete Blood Count and Bone Marrow Examination: General Comments and Selected Techniques Graphic displays of cells aid in diagnosis (Tvedten, Scott, and Boon, 2000).
Monocytes have a little peroxidase and are located between the neutrophils and lymphocytes. Platelets, platelet clumps, and cell debris are very small and at the lower left. Large unstained cells (LUC) are at the upper left. LUC is not a cell type but a name used for cells of certain staining and size characteristics. LUC are not found in the other identifiable cell clusters. LUC include large lymphocytes, monocytes, and blast cells. The basophil cytogram has cell size on the vertical axis and nuclear density on the horizontal axis.